Figure 4

Comparison of the level of exogenous hBST-2 on plasma membrane with its inhibition efficiency for HIV-1 release in various cell lines. (A and B) The results shown in Figures 2 and 3 were summarized and rearranged as follows: the level of surface expression of hBST-2 is shown in MFI (A) and the percentage of surface hBST-2 positive cells (B) in the X-axis. To calculate % virus release (Y-axis), the infectivity of the culture supernatant of phBST-2-untransfected cells (for HEK293, Vero, Cos-7, D-17, RSC, Mv.1.Lu, and QT6 cells) or the amount of p24 in the culture supernatant of phBST-2-untransfected cells (for CRFK and PtK2) was defined as 100%. Statistical significance of the correlation between the level of surface hBST-2 (X-axis, shown in MFI or % positive cells) and % virus release (Y-axis) in the results from the 9 analyzed cells (HEK293, Cos-7, D-17, CRFK, PK-15, RSC, Mv.1.Lu, PtK2, and QT6 cells) was determined by Pearson's correlation test, and P < 0.01 was considered significant. Approximation curve of the result from the 9 analyzed cells is drawn in gray lines, and a representative result from Vero cells is drawn in broken line. r, Pearson's correlation coefficient.