Figure 2

Circular dichroism (CD) spectra of Tat variants in aqueous solution. Tat Z2 (white triangle), Tat Oyi (black triangle), Tat Bru (white circle), Tat Bru cmC (no mark), Tat Jr (black circle), Tat Mal (white square) and Tat Eli (black square) were measured from 260 to 178 nm with a 50 μM path length in 20 mM phosphate buffer, pH 4.5. It is not possible to gather CD spectra into two categories composed of short Tat (white mark) or long Tat (black mark). The intense magnitude of the 200 nm band observed with Tat Bru cmC shows that chemical modifications of cysteines modify the folding of Tat.