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Figure 4 | Retrovirology

Figure 4

From: Microarray study reveals that HIV-1 induces rapid type-I interferon-dependent p53 mRNA up-regulation in human primary CD4+T cells

Figure 4

Virus-induced type-I IFN secretion and blocking experiments with anti-IFN-Ī± and -Ī² antibodies. (A) Cells were either left uninfected or infected with NL4-3 wt for the indicated times. Thereafter, .HEK-Blueā„¢ IFN-Ī±/Ī² cells were exposed to the collected cell-free supernatants for 24 h and type-I IFN levels were quantified according to manufacturer's instructions. (B) The purified cell population highly enriched in CD4+ T lymphocytes was either left uninfected or infected with NL4-3 wt for 24 h. In some samples, neutralizing antibodies against human IFN-Ī± and IFN-Ī² were added in equal quantities (a final concentration of 1 Ī¼g/ml was used). Controls consisted of cells treated with isotype-matched irrelevant antibodies (IgG). Thereafter, total RNA was isolated and p53 mRNA levels were estimated by qRT-PCR. The data shown represents the mean Ā± standard deviations of triplicate samples and are representative of three independent experiments. Asterisks denote statistically significant differences from the uninfected control cells (*, P < 0.05; **, P < 0.01; ***, P < 0.001).

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