Figure 3

Quantification of p53 by qRT-PCR and western blot. (A) The purified cell population highly enriched in CD4⁺ T lymphocytes was either left uninfected or infected with NL4-3 wt for 8, 24, 48 and 72 h. Total RNA from the five original healthy donors and from six additional donors was isolated. Ribosomal 18S and p53 RNA levels were quantified by qRT-PCR. Data shown is representative of all studied samples (i.e. a total of eleven) normalized on ribosomal 18S. (B) Cells were either left uninfected (mock) or infected with NL4-3 wt for the indicated time periods. Next, p53 and actin protein levels were estimated by western blot analysis using a specific antibody.