Quantification of p24 production in the total T cell fraction and CD4+CD8- T cell fraction derived from hCycT1/CRM1 Tg rats. Stimulated spleen-derived T cells from WT or Tg rats and hPBL were electroporated with 4 μg PCRRE and 1 μg pMax-GFP, and p24 production in the supernatants (A) and cell lysates (B) was measured by ELISA (left panel). The percentage of living cells was 30–40%, and 28–40% of the living cells were GFP+. The right panels represent the fold activation of Tg versus WT rats. Stimulated CD4+CD8- T cells derived from WT, hCycT1/CRM1 Tg rats, and human blood were electroporated, as above, and p24 production in the supernatants (C) and cell lysates (D) was measured. The percentage of living cells was ~10%, and 30–40% of the living cells were GFP+. Values are the means of duplicate samples.