Figure 5
From: Synergistic effect of human CycT1 and CRM1 on HIV-1 propagation in rat T cells and macrophages
Characterization of hCycT1 and hCRM1 Tg rats. (A) The expression of hCycT1 in spleen- and thymus-derived cells from WT or hCycT1 Tg rats was confirmed by Western blotting using anti-hCycT1. (B) T cells derived from the spleen of WT or hCycT1 Tg rats were stimulated with anti-rat-CD3 and anti-rat-CD28. Cells were collected at the indicated times and subjected to Western blotting using anti-hCycT1. (C) The expression of hCycT1 and hCRM1 in spleen- and thymus-derived cells (C), total T and CD4+CD8- T cells (D), and macrophages (E) in WT or Tg rats was confirmed by Western blotting using anti-hCycT1 and anti-hCRM1. T cells derived from the spleen of WT or hCycT1 Tg rats were stimulated with anti-rat-CD3 and anti-rat-CD28.