Figure 3

Synergistic effect of hCycT1 and hCRM1 in rat macrophage cell lines. (A) NR8383 cells were electroporated as described in Fig. 1B. The percentage of living cells was approximately 20–40%, and approximately 75% of the living cells were GFP⁺. The amount of p24 in the sample containing hCRM1-HA was 196 pg. Approximately 50 μg samples of the cell lysates were subjected to Western blotting as described in the Methods. (B) NR8383 cell lines were electroporated as described in Fig. 1A. The percentage of living cells was approximately 15%, and approximately 60% of the living cells were GFP⁺. The amount of p24 in the sample containing hCRM1-HA/hCycT1 was 56 pg. (C) NR8383 cell lines were electroporated with 2 μg pΔpol, 0.4 μg pH1-Luc and 0.2 μg pCDMβ-gal along with or without 1 μg pβhCycT1 and 0.5 μg pSRαhCRM. pSRα296 was added to adjust the total amount of the plasmids. The amounts of p24 in the cell lysate and medium of the sample containing hCRM1/hCycT1 were 488 and 96 pg, respectively. Values are means of triplicate samples.