Effect of hCycT1 and hCRM1 expression in rat T cell lines (part 2). (A) FPM1 and (B) C58(NT)D cells were electroporated, as above, with the exception that 0.4 μg pH1-Luc and 0.2 μg pCDMβ-gal were used instead of pMax-GFP. LTR activity and transfection efficiency were measured by luciferase and β-gal assays using cell lysates. The luciferase/β-gal activity or the amount of p24 was calculated, and the value of the mock sample was normalized to 1. Values are means of triplicate samples and the SD was calculated. The amount of p24 in the FPM1 and C58(NT)D samples containing hCycT1/hCRM1 was 3.7 and 2.8 ng, respectively. (C) FPM1 cells continuously expressing hCycT1 and hCRM1 were electroporated with 4 μg pNL4-3 and 1 μg pMaxGFP. The percentage of living cells was approximately 10%, and 50% of the living cells were GFP+. The amount of p24 in the FPM1hCT/hCRM1 sample was 6.0 ng. Approximately 10 μg of each cell lysate were subjected to Western blotting.