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Figure 5 | Retrovirology

Figure 5

From: TLR2 and TLR4 triggering exerts contrasting effects with regard to HIV-1 infection of human dendritic cells and subsequent virus transfer to CD4+T cells

Figure 5

TLR2 stimulation influences an early step in HIV-1 life cycle. A)IM-MDDCs were either left untreated (mock) or stimulated for 2 hours with the TLR2 agonist Pam3Csk4 (5 μg/ml) before or after exposure for 1 hour to NL4-3/Balenv. Supernatants were harvested at 72 hours post-infection and the viral content was evaluated by a p24 test. B) Cells were infected with VSV-G pseudotyped reporter viruses for 2 hours, washed twice and put in culture for 48 hours. Next, IM-MDDCs were either left untreated (mock) or stimulated for 2 hours with Pam3Csk4. Cells were then washed twice, cultured for 48 hours and lysed to monitor luciferase activity (expressed in relative light units/RLU). The data shown represent the mean ± standard deviations of quadruplicate samples from a single donor and are representative of 3 distinct donors.

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Retrovirology

ISSN: 1742-4690

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