Analysis of transcribed human endogenous retrovirus W env loci clarifies the origin of multiple sclerosis-associated retrovirus envsequences

Table 2 Recombined HERV-W env cDNA sequences generated in this study

Recombination events	Number of involved loci	Number of cDNA sequences	Sum of involved HERV-W env transcripts
0	1	233	233
1	2	66	132
2	2	11	33
2	3	16	48
3	3	2	6
3	4	1	4
4	5	3	15
		Σ = 332	Σ = 471

Recombinations were detected by inspection of multiple alignments of cloned HERV-W env cDNA sequences and transcribed genomic HERV-W env loci identified in this work (for details, see Methods). Altogether, 99 out of 332 (29.8%) cDNA sequences analyzed represented recombined sequences. The sum of RNA transcripts from which recombined cDNA orignated is given in the right column. In the case that multiply recombined sequences involved the same locus more than once, it was assumed that sequences from the same locus originated from different transcripts from that locus.

ISSN: 1742-4690