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Table 5 Competitive transfer rates of PFV (TW05, ref fluorescence) and SFVmac (EGFPD, green fluorescence) vectors following simultaneous packaging into PFV or SFVmac particles.

From: Accuracy estimation of foamy virus genome copying

vectors and packaging systems

fluorescence-positive cells

competitive cross-packaging efficiency

PFV vector (red) +

68% red cells

---

PFV gag pol

  

SFVmac vector (green) +

67% green cells

---

SFVmac gag pol

  

PFV vector (red) +

63% red cells

---

SFVmac vector (green) +

0,85% green cells

1.3%

PFV gag pol

  

PFV vector (red) +

6,8% green cells

---

SFVmac gag pol +

1,2% red cells

15%

SFVmac vector (green)

  
  1. Vectors were packaged together with the indicated packaging proteins in 293T cells. Supernatants from packaging cells were used for transduction of HT1080 cells. Transduction rates were measured by detection of EGFP and mRFP expression by flow cytometry. The competitive cross-packaging efficiency is calculated from the ratio of fluorescence-positive cells of the heterologous system versus the homologous + heterologous system (e.g. 1.3% = 0.85%/(63% + 0.85%)).