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Table 1 PFV point mutations identified after a single round of replication in the absence or presence of Bet.

From: Accuracy estimation of foamy virus genome copying

  vector packaging in 293T cells w/o Bet vector packaging in 293T cells with Bet
type of mutation number of mutations in a total of 93,003 nucleotides number of mutations in a total of 172,368 nucleotides
G→A 39 35
G→T 0 0
G→C 0 0
A→G 0 2
A→T 0 0
A→C 0 0
T→G 0 0
T→A 0 0
T→C 0 0
C→G 0 0
C→A 0 0
C→T 0 0
total 39 37
error rate 4.2 × 10-4 2.1 × 10-4
  1. Vector particles were packaged in HEK 293T cells in the absence or presence of a bet expression plasmid. Vector preparations were used to transduce HeLa cells. Singly transduced cells were sorted into 96 well plates to form monoclonal cell cultures that carry a single provirus. Proviral DNA was amplified by PCR and sequenced in order to assess the in vivo error rate of FV replication.