Accuracy estimation of foamy virus genome copying

Retrovirology

Table 1 PFV point mutations identified after a single round of replication in the absence or presence of Bet.

	vector packaging in 293T cells w/o Bet	vector packaging in 293T cells with Bet
type of mutation	number of mutations in a total of 93,003 nucleotides	number of mutations in a total of 172,368 nucleotides
G→A	39	35
G→T	0	0
G→C	0	0
A→G	0	2
A→T	0	0
A→C	0	0
T→G	0	0
T→A	0	0
T→C	0	0
C→G	0	0
C→A	0	0
C→T	0	0
total	39	37
error rate	4.2 × 10^-4	2.1 × 10^-4

Vector particles were packaged in HEK 293T cells in the absence or presence of a bet expression plasmid. Vector preparations were used to transduce HeLa cells. Singly transduced cells were sorted into 96 well plates to form monoclonal cell cultures that carry a single provirus. Proviral DNA was amplified by PCR and sequenced in order to assess the in vivo error rate of FV replication.

ISSN: 1742-4690