HIV-resistance in CEM.NKR is not due to the loss of calnexin expression. A) Transduction of calnexin gene into CEM.NKR cells. Calnexin (CANX) or a control (GFP) gene with a 3'-HA tag was cloned into the pMSCVneo retroviral vector. Recombinant retroviruses were produced by transfection of the Phoenix-AMPHO cell line and were used to infect CEM.NKR, CEM-T4, and CEM-SS cells. Four stable cell lines were created by G418 selection and the expressions of transduced genes were determined by Western blotting using an anti-HA antibody. B) HIV-1 (NL4-3) replication in these four cell lines.