Figure 3
Activity and expression of A3 cytidine deaminases in cell clones from CEM.NKR. A) Wild-type and vif-defective HIV-1 replication in CEM.NKR cell subpopulations. A total of 2 × 105 of CEM-T4, CEM.NKR, and the eight clones were infected with 150 ng p24Gag of wild-type or vif-defective HIV-1 (NL4-3) and viral production was measured for 16 days. B) A3G and A3F protein expression in CEM.NKR and its cell clones determined by Western blotting. C) A3DE and A3H mRNA expression in CEM.NKR and its cell clones as determined by real-time PCR. Results are presented as relative values, where expression levels in H9 cells are set as 100%.