Localization of HIV-1 Gag and Env with tetraspanins in permeabilized HIV-1 infected MOLT cells. MOLT/HIV-1 cells were fixed, permeabilized, and stained with the anti-tetraspanin CD9, CD63, CD81 or CD82 antibodies, or with antibodies against CD45 or Lamp2. To reveal the viral proteins Gag and Env, the cells were co-stained with anti-MAp17 (Gag in green) and anti-SU gp120 (Env in red) antibodies. The percentage of Gag co-localization with the markers was calculated by image analysis and reported in the graph (Fig. 3).