Figure 5

Disruption of P-bodies through knockdown of RCK/p54 and LSm-1 leads to enhanced production of infectious HIV-1 virions. HeLa CD4+ cells were transfected with siRNA as indicated. 48 hours post transfection cells were analyzed for RCK/p54 and LSm-1 expression by Western blotting (a) and infected with equal amounts of HIV-1 (200 ng/ml). b) Virus production was monitored 48 hours post infection by measuring p24 antigen in culture supernatant. c) To analyze the infectivity of new progeny virions, equal volumes of supernatant from siRNA transfected Hela CD4+cells were used to re-infect HeLa CD4+ cells. P24 antigen was measured in culture supernatant 48 hours post infection.