Figure 1

miRNA effectors are repressors of HIV-1 replication. HeLa cells were transfected with siRNA as indicated. 48 hours post transfection, cells were analyzed for RCK/p54, LSm-1, GW182, XRN1, DGCR8, DROSHA and CDK9 expression by Western blotting (a), or infected with a single round infectious virus (HIV-1-VSV-luc 200 ng/ml) and cell extracts were measured for luciferase activity 48 hours after infection (b). Results are presented as fold HIV production relative to Scr transfected cells, and data are representative of three independent experiments.