Nucleic acids content of wild-type and NC mutant HIV-1 virions. WT, ΔZF1, ΔZF2 and ΔZF1ZF2 represent wild type, and deletion of the first, the second and of both CCHC zinc fingers, respectively. All values were determined by quantitative RT-PCR (A) and PCR (B) (n = 3 ± SD). A- Viral RNAs corresponding to the full length (FL) and the multispliced (MS) forms. B- Viral DNAs corresponding to the strong stop (ss), Gag, full length (FL) and mutispliced (MS) forms.