Table 2 Primers and probes used to quantify viral sequences by real-time PCR
From: Modulation of HIV-1 infectivity and cyclophilin A-dependence by Gag sequence and target cell type
GAPDH | |
forward primer | 5'- ACCCCTGGCCAAGGTCATC |
reverse primer | 5'- AGGGGCCATCCACAGTCTTC |
Probe | 5'- (6-Fam)AGGACTCATGACCACAGTCCATGCCA(Tamra) |
CypA | |
forward primer | 5'- GGCCGCGTCTCCTTTGA |
reverse primer | 5'- AATCCTTTCTCTCCAGTGCTCAGA |
Probe | 5'- (6-Fam)TGCAGACAAGGTCCCAAAGACAGCAG(Tamra) |
TRIM5α | |
forward primer | 5'- TGCCTCTGACACTGACTAAGAAGATG |
reverse primer | 5'- GGGCTAAGGACTCATTCATTGG |
Probe | 5'- (6-Fam)AAGCTTTTCAACAGCCTTTCTATATCATCGTGTGATA(Tamra) |