hCycT1 transgenesis boosts early HIV gene expression in infected primary T-cells, but not in macrophages. (A, B)Activated T-cells from hCycT1-tg and n-tg rats were infected with VSV-G HIV-1NL4-3 or HIV-2ROD-A GFP reporter viruses and analyzed by flow cytometry three days later. (B, C) Cumulative results from several independent infection experiments of cells derived from rats or human donors. Closed circles depict the MFI of (B) HIV-infected T-cells or (C) HIV-infected macrophages as the mean of triplicates of experiments performed for cultures from individual donors. Open triangles represent the arithmetic mean of the MFI of all donors in one group ± SEM. n.s. = not significant.