hCycT1 transgenensis enhances the Tat-responsiveness of rat T-cells. Activated, spleen-derived T-cells from three hCycT1-tg and three n-tg rats were nucleofected with (A, B) minimal reporter constructs pHIV-1NL4-3 LTR GFP or pHIV-2ROD-A LTR GFP in the presence or absence of HIV-1 Tat or HIV-2 Tat expression constructs, respectively, or (C, D) full-length proviral GFP reporter constructs pHIV-1NL4-3 GFP or pHIV-2ROD-A. Analysis of gene expression was performed as in Fig. 2. (E, F) In addition, T-cell cultures from the same rats were infected with corresponding VSV-G pseudotyped HIV-1NL4-3 GFP or HIV-2ROD-A GFP viruses and analyzed for reporter gene expression by flow cytometry three days later. Given are the arithmetic mean + SD of triplicates.