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Figure 10 | Retrovirology

Figure 10

From: Human cyclin T1 expression ameliorates a T-cell-specific transcriptional limitation for HIV in transgenic rats, but is not sufficient for a spreading infection of prototypic R5 HIV-1 strains ex vivo

Figure 10

hCycT1 transgenesis boosts replication of HIV-1 R7/3 YU-2 Env GFP in T-cells from multi-tg rats. Activated T-cells derived from the spleens of four double- or four triple-tg rats were challenged with (A) single-round VSV-G HIV-1NL4-3 GFP reporter virus and the MFI (GFP) of viable cells was quantified on day 3 p.i. (B) In parallel, T-cell cultures were challenged with HIV-1R7/3 YU-2 Env GFP either in the absence (circles) or presence (triangles) of efavirenz (5 μM). Cultures were washed the following day and cultivated for 14 days. At the indicated time points p.i., the percentage of GFP-positive cells was determined by flow cytometry. Shown are the arithmetic mean ± SD of triplicates. In the group of hCD4/hCCR5/hCycT1-tg rats significantly higher replication levels were reached compared to the group of hCD4/hCCR5-tg rats (p = 0.02; linear model variance analysis).

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Retrovirology

ISSN: 1742-4690

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