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Figure 1 | Retrovirology

Figure 1

From: Human cyclin T1 expression ameliorates a T-cell-specific transcriptional limitation for HIV in transgenic rats, but is not sufficient for a spreading infection of prototypic R5 HIV-1 strains ex vivo

Figure 1

Expression of hCycT1 in CD4 T-cells and macrophages from hCycT1-tg rats. (A) Schematic representation of the tg vector for hCycT1, pMΦE4A.CyclinT1. (For details, see "Methods" and [25]. E4/P4: murine CD4 enhancer/promoter. (B) hCycT1 transgene-specific PCR amplifying a diagnostic 1.7-kb fragment in tail DNA from hCycT1-tg rats. M: DNA marker. (C-E) Western blot analysis of hCycT1 expression in extracts from (C) thymocytes, (D) spleen-derived CD4 T-cells and CD8 T-cells, enriched by magnetic bead selection, or (E) spleen-derived macrophages, using an antibody specific for CycT1 of human origin. Cells from n-tg littermates, or human Sup-T1 T-cells and human MDMs served as negative and positive controls, respectively. (C, D) Blots were reprobed for ERK2 as loading reference. (D) * indicates the hCycT1-specific band. The lower band seen in all splenocyte samples was considered non-specific. # gives the ID numbers of individual tg rats.

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Retrovirology

ISSN: 1742-4690

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