Figure 6
From: HIV-1 TAR miRNA protects against apoptosis by altering cellular gene expression
Specifically blocking TAR miRNA sensitized cells to apoptosis. HLM-1 cells were transfected with antagomir complementary to the TAR 5' miRNA. Twenty-four hours post transfection the media was replaced with DMEM with 0.1% FBS. Cells were sampled at 96 hours and apoptosis determined via AnnexinV and PI co-staining. Percentages shown indicate the number of AnnexinV positive, PI negative and AnnexinV, PI double positive cells.