Figure 5
From: HIV-1 TAR miRNA protects against apoptosis by altering cellular gene expression
Serum starvation induced cleavage of Caspase 3 in uninfected but not infected cells. (A) HeLaT4 (lanes 1 and 3) and HLM-1 (lanes 2 and 4) were Western blotted for Caspase 3 expression and cleavage at Zero (lanes 1 and 2) and 48 (lanes 3 and 4) hours after serum starvation. (B) CEM (lanes 1 and 3) and ACH2 (lanes 2 and 4) were Western blotted for Caspase 3 expression and cleavage at Zero (lanes 1 and 2) and 48 (lanes 3 and 4) hours after serum starvation. Densitometry was performed to determine the density of the cleaved 17 and 20 kDa Caspase 3 bands as compared to the 32 kDa inactive form.