Figure 1
From: HIV-1 TAR miRNA protects against apoptosis by altering cellular gene expression
Determination of the sequence of that HIV-1 TAR miRNA. RNA from cMagi cells infected with HIVIIIB was used to construct miRNA libraries and used for cloning. (A) Cloned sequences of the TAR-5p and TAR-3p (5' and 3' arm) miRNA obtained as compared to predicted sequence registered with the Sanger miRNA database. (B) Diagram showing the TAR hairpin and the position of the mature miRNA within the TAR sequence. (C) Structure of the TAR-WT and truncated TAR-D mutant used for 293T transfections. (D) 293T cells were mock transfected (lane 2) or transfected with TAR-WT (lane 3) or TAR-D (lane 4) RNA. Forty-eight hours after transfection RNA was isolated and subjected to Northern blotting for TAR sequence. Numbers to the left indicate the size of the RNA ladder in nucleotides. Diagrams to the right show the positions of the wild-type TAR and the mature TAR miRNA.