Figure 7

Degradation of HML-2 SP by the proteasome. HeLa cells expressing EnvΔRec or Env were incubated, 16 hours post-transfection, with proteasome inhibitor MG132 at 10 μM. Cells were collected every hour for, in total, 8 hours and analyzed by Western blotting using anti-SP. Cellular β-actin was analyzed as a loading control. Larger-sized protein bands, including the protein band slightly larger than SP in the left-hand Western blot, correspond to cytosolic translation products that are due to saturation effects (see text).