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Figure 6 | Retrovirology

Figure 6

From: Human endogenous retrovirus HERV-K(HML-2) encodes a stable signal peptide with biological properties distinct from Rec

Figure 6

Determination of half-life of HML-2 SP. HeLa cells expressing either EnvΔRec or Env were incubated, 16 hours post-transfection, with the translation inhibitor CHX at 100 μg/ml. SP and Rec molecules were chased for up to 8 hours. Every hour, cells were collected and analyzed by Western blot using anti-SP. Cellular β-actin was analyzed as a loading control. Larger-sized protein bands correspond to cytosolic translation products due to saturation effects (see text).

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