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Table 2 Dinucleotide context of G-to-A mutations in Vif/Vpr and DIS/Gag regions.

From: APOBEC3G induces a hypermutation gradient: purifying selection at multiple steps during HIV-1 replication results in levels of G-to-A mutations that are high in DNA, intermediate in cellular viral RNA, and low in virion RNA

Virus (Region sequenced)

Dinucleotide context of G-to-A Mutations

 

GGa (%)

GA (%)

GC (%)

GT (%)

Total

WT (Vif/Vpr)

     

   DNA

1 (25%)

3 (75%)

0

0

4

   Cellular viral RNA

0

0

0

0

0

   Virion RNA

7 (32%)

11 (50%)

1 (5%)

3 (14%)

22

HIV-YRHHY>A5 (Vif/Vpr)

     

   DNA

620 (87%)

83 (12%)

6 (1%)

3 (0.4%)

712

   Cellular viral RNA

81 (86%)

9 (10%)

3 (3.2%)

1 (1%)

94

   Virion RNA

34 (69%)

10 (20%)

3 (6%)

2 (4%)

49

HIV-YRHHY>A5 (DIS/Gag)

     

   DNA

54 (87%)

8 (13%)

0

0

62

   Cellular viral RNA

74 (89%)

3 (4%)

3 (4%)

3 (4%)

83

   Virion RNA

20 (74%)

4 (15%)

0

3 (11%)

27

  1. a The first G nucleotide in the GG dinucleotide is the target of G-to-A mutation.