APOBEC3G induces a hypermutation gradient: purifying selection at multiple steps during HIV-1 replication results in levels of G-to-A mutations that are high in DNA, intermediate in cellular viral RNA, and low in virion RNA

Table 1 Infectivity of HIV WT and HIV-YRRHHY > A5 virus-containing supernatants from samples with peak RT activities.

Virus	Round of Infection^a	Relative Infectivity^b (%) ± S.D.^c	Fold Decrease in Infectivity
WT	1	100 ± 4.5	-
YA	1	11.6 ± 0.2	8.6
YB	1	13.6 ± 0.1	7.4
YC	1	14.2 ± 0.1	7.0
WT	2	100 ± 3.0	-
YA	2	10.6 ± 0.5	9.5
YB	2	7.3 ± 0.5	13.7
YC	2	4.6 ± 0.7	21.7
WT	3	100 ± 4.2	-
YA	3	0.9 ± 0.0	106.4
YB	3	5.2 ± 1.0	19.1
YC	3	2.2 ± 0.4	44.7

^a Peak time points analyzed are shown in Fig. 2.
^b The infectivity of equivalent volumes of cell supernatants was assessed using TZM-bl cells. Luciferase activities in infected cell lysates were measured 24 hours after infection.
^c S.D., standard deviation.

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