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Table 1 Steady state kinetic analysis for dATP and TFV-DP: measurement of relative inhibitory capacity (Ki/Km ratio)

From: Effects of the K65R and K65R/M184V reverse transcriptase mutations in subtype C HIV on enzyme function and drug resistance

HIV-1 RT Enzyme

Km(dATP), μMa

Ki(TFV), μMb

Ki/Km(fold)c

Subtype C WT

0.51 ± 0.04

0.24 ± 0.04

0.47(1.0)

Subtype C K65R

0.64 ± 0.05

2.2 ± 0.07*

3.43(7.2)

Subtype C K65RM184V

0.83 ± 0.04*

1.2 ± 0.05*

1.93(4.1)

  1. a Km and b Ki values are mean values from at least three experiments ± SD.
  2. c Fold change in Ki/Km from wild-type.
  3. * P ≤ 0.01 compared to the wild-type by two-tailed Student's t-test.
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Retrovirology

ISSN: 1742-4690

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