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Table 1 Steady state kinetic analysis for dATP and TFV-DP: measurement of relative inhibitory capacity (Ki/Km ratio)

From: Effects of the K65R and K65R/M184V reverse transcriptase mutations in subtype C HIV on enzyme function and drug resistance

HIV-1 RT Enzyme Km(dATP), μMa Ki(TFV), μMb Ki/Km(fold)c
Subtype C WT 0.51 ± 0.04 0.24 ± 0.04 0.47(1.0)
Subtype C K65R 0.64 ± 0.05 2.2 ± 0.07* 3.43(7.2)
Subtype C K65RM184V 0.83 ± 0.04* 1.2 ± 0.05* 1.93(4.1)
  1. a Km and b Ki values are mean values from at least three experiments ± SD.
  2. c Fold change in Ki/Km from wild-type.
  3. * P ≤ 0.01 compared to the wild-type by two-tailed Student's t-test.