Figure 5
From: HIV-1 exploits importin 7 to maximize nuclear import of its DNA genome
Imp7 KD impacts on the efficiency of HIV-1 nuclear import. DxR and imp7 KD HeLa cells were infected with an HIV-1 vector (pCSGW) at an MOI of approximately 0.1 and analyzed at the indicated time points by (A) flow cytometry to measure GFP+ cells, (B) Taqman PCR to measure total viral DNA copy number, (C) Taqman PCR to measure 2LTR circular DNA copy number. Data are expressed as mean value ± SD of three independent experiments. Statistical significance was calculated by the Student t-test (n = 3), ** p < 0.005.