Viral replication and dissemination in the tissues of macaques during primary infection with SIVmac251. The three viral markers -- viral RNA, DNA and 2 LTR circles -- were evaluated in various tissues from macaques infected with SIVmac251, on days 14, 21, 28 and 100 pi. The relative level of viral RNA with respect to the mRNA for GAPDH was calculated by the "delta delta Ct" method. Absolute copy numbers for viral DNA and 2LTR circles were calculated to the GAPDH and normalized to one million of cells. When significant, p values were indicated. The results from blood were added to tissues as comparative value.