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Figure 2 | Retrovirology

Figure 2

From: Phosphorylation regulates human T-cell leukemia virus type 1 Rex function

Figure 2

Functional activity and expression of S-tagged Rex-1. (A) The functional activity of S-tagged Rex-1 was determined using an HIV p24 Gag reporter assay. 293T cells were transfected with 0.25 μg pcTat, 0.5 μg pcGagRxRE-I, 0.05 μg CMV-luc, and 0.1 μg of wtRex-1 or S-Rex-1 expression plasmids. Twenty-four hours post-transfection, cells were harvested and assayed for p24 Gag. The values represent actual p24 Gag production from a representative experiment performed in triplicate. Error bars indicate standard deviations. (B) Affinity purification of S-tagged Rex-1 from mammalian cells. 293T cells were transfected with S-Rex-1 and S-tagged Rex-1 was purified by S-protein-agarose beads, eluted and resolved by SDS-PAGE analysis, and detected by Coomassie blue staining.

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