Generation of ROS in B lymphocyte mitochondria. Confocal microscopy analysis of ROS production in B cells isolated from 3 BLV-infected and 3 control sheep. 24 h after culture, PBMCs were labeled with 10 nM of Red CM-XROS probe and stained with anti-IgM monoclonal antibody (clone Pig45) and Alexa Fluor 488 goat anti-mouse conjugate. A) Confocal microscopy photographs and profiles of one representative experiment showing the fluorescence intensities of ROS (red fluorescence) and IgM (green fluorescence). For comparison, arrows indicate ROS levels (red) in B cells (green) in PBMCs from infected and control sheep. B) Quantification of arbitrary fluorescence intensities in 20 B cells from 3 BLV- infected and 3 control sheep. Data are presented as the means of fluorescence intensities (± standard deviation). *** denotes the statistical significance according to the non paired Student's t test p < 0.001.