Figure 2
From: Multi-stage Friend murine erythroleukemia: molecular insights into oncogenic cooperation
Erythroleukemia in spi-1 transgenic mice is a two-step disease. The first step occurring in spi-1 transgenic mice is a massive proliferation of proerythroblasts, arrested in differentiation around the CFU-E/proerythroblast stage. These cells can be established in vitro as Epo-dependent cell lines. In a second step, malignant proerythroblastic cells emerge that proliferate in vitro without Epo and are tumorigenic when grafted into Nude mice.