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Figure 5 | Retrovirology

Figure 5

From: Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene

Figure 5

A) FISH for HMBOX1 RNA on parental HOS_143b cells. Top panel: large field image (bar = 10 μm). Bottom panels: single cell from the figure above (inset). Two distinct hybridization signals per nucleus demonstrate bi-allelic expression of the HMBOX1 gene. B) Same as A after transfection of Tat-EGFP. Also the signal for Tat-EGFP is shown (middle panels). Bottom panel: merge of HMBOX1 hybridization and Tat-EGFP expression. C) FISH for HIV-Intro RNA on HOS_A4 cells. Top panel: large field image (bar = 10 μm). Bottom panels: single cell from the figure above (inset). Absence of the hybridization signal with the HIV probe is due to silencing of the gene without Tat (left panels). Two distinct hybridization signals per nucleus demonstrate bi-allelic expression of the HMBOX1 gene (middle panels).

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