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Figure 4 | Retrovirology

Figure 4

From: Intragenic transcriptional cis-activation of the human immunodeficiency virus 1 does not result in allele-specific inhibition of the endogenous gene

Figure 4

A) RT-PCR analysis of HMBOX1 expression in parental HOS_143b and clone HOS_A4 using primers for HMBOX1 Exon 3 (79 bp, top panel) and Exon 2/Exon 3 splicing (86 bp, middle panel). Bottom panel: β-actin control (230 bp). B) RT-PCR analysis of HIV-Intro expression (280 bp top panel) in parental HOS_143b and clone HOS_A4 using primers HIV_SPLICED and HIV_NUC (Table 1). Bottom panel: β-actin control (M = molecular weight marker; T = hours after Tat induction). C-H) Quantitative RT PCR for HIV-Intro, HMBOX1 and GAPDH expression using the indicated primers shown in Figure 1 and Table 1. Each histogram is the mean of three experiments normalized for β-actin expression and corrected for primer efficiency (E = 10-1/slope).

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