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Figure 3 | Retrovirology

Figure 3

From: Cofilin activation in peripheral CD4 T cells of HIV-1 infected patients: a pilot study

Figure 3

Lack of detection of T cell activation markers on the surface of resting CD4 T cells from HIV-1-infected and uninfected donors. Resting CD4 T cells were purified by antibody-mediated negative depletion using Dynalbeads. Cells purified from HIV-1-infected and uninfected donors were cultured overnight in the absence of cytokines or activation, and then stained for surface expression of HLA-DR or CD69. Shown are flow cytometry analyses of cells stained with a PE-labelled anti-human HLA-DR antibody (A and C, right panel) or a similarly labelled isotype control antibody (A and C, left panel). Cells were also stained with a PE-labelled anti-human CD69 antibody (B and D, right panel) or a similarly labelled isotype control antibody (B and D, left panel). Cells from the HIV negative donor (HIV-) were used in (A) and (B), and cells from the HIV-1-infected donor (HIV+) were used in (C) and (D).

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