Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model

Ohashi, Takashi; Nagai, Mika; Okada, Hiroyuki; Takayanagi, Ryo; Shida, Hisatoshi

doi:10.1186/1742-4690-5-90

Table 1 Primers to construct SCTs of RT1.A¹

From: Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model

Constructs		First PCR	Second PCR*	Third PCR*	Forth PCR
RT1AlSCTax180S	Sense primer	ATTCAGAAAACTC CCCAAATTCAAG TGTAC	GGCCGCCCTGGCCCCGACCCAGACC CGCGCGGGGGCCTTCCTCACCAATG TTCCCTACGGAGGTGGCGGGTCCGG AGGTGGCGGGTCCATTCAGAAAACT CCCCAAATTCAAGTGTACTCTCGCC ATCCA	CCTGCTGCTGGCGGCC GCCCTGGCC CCGAC	Not applicable
	Reverse primer	CGCCACCTCCCAT GTCTCGGTCCCA GGTGA	CCGAGGCCGGGCCGGGACACGGCGA TGTCGAAATACCGCATCGAGTGAGA GCCGGACCCGCCACCTCCGGACCCG CCACCTCCGGACCCGCCACCTCCCA TGTCTC	CCGGGGCTCCCCGAG GCCGGGCCGGGACAC GGCGATGTC	Not applicable
RT1AlSCNLEnv371S	Sense primer	ATTCAGAAAACTC CCCAAATTCAAG TGTAC	GGCCGCCCTGGCCCCGACCCAGACC CGCGCGCACAGTTTTAATTGTGGAG GGGAATTTGGAGGTGGCGGGTCCGG AGGTGGCGGGTCCATTCAGAAAACT CCCCAAATTCAAGTGTACTCTCGCC ATCCA	CCTGCTGCTGGCGGCC GCCCTGGCCCCGAC	Not applicable
	Reverse primer	CGCCACCTCCCAT GTCTCGGTCCCA GGTGA	CCGAGGCCGGGCCGGGACACGGCGA TGTCGAAATACCGCATCGAGTGAGA GCCGGACCCGCCACCTCCGGACCCG CCACCTCCGGACCCGCCACCTCCCA TGTCTC	CCGGGGCTCCCCGAG GCCGGGCCGGGACAC GGCGATGTC	Not applicable
RT1AlSCTax180L	Sense primer	ATTCAGAAAACTC CCCAAATTCAAG TGTAC	GGAGGTGGCGGGTCCATTCAGAAAA CTCCCCAAATTCAAG	GGCCGCCCTGGCCCCG ACCCAGACCCGCGCG GGGGCCTTCCTCACC AATGTTCCCTACGGA GGTGGCGGGTCCGGA GGTGGCGGGTCCGGA GGTGGCGGGTCC	CCTGCTGCTGGCGGC CGCCCTGGCCCCGAC
	Reverse Primer	CGCCACCTCCCAT GTCTCGGTCCCA GGTGA	GGCCGGGACACGGCGATGTCGAAAT ACCGCATCGAGTGAGAGCCGGACCC GCCACCTCCGGACCCGCCACCTCCG GACCCGCCACCTCCGGACCCGCCAC CTCCCATGTCTC	CCGGGGCTCCCCGAG GCCGGGCCGGGACAC GGCGATGTC	GCTCCCCGAGGCCGG GCCGGGACACGGCGA
RT1AlSCNLEnv371L	Sense Primer	ATTCAGAAAACTC CCCAAATTCAAG TGTAC	GGAGGTGGCGGGTCCATTCAGAAAA CTCCCCAAATTCAAG	GGCCGCCCTGGCCCCG ACCCAGACCCGCGCG CACAGTTTTAATTGT GGAGGGGAATTTGGA GGTGGCGGGTCCGGA GGTGGCGGGTCCGGA GGTGGCGGGTCC	CCTGCTGCTGGCGGC CGCCCTGGCCCCGAC
	Reverse Primer	CGCCACCTCCCAT GTCTCGGTCCCA GGTGA	GGCCGGGACACGGCGATGTCGAAAT ACCGCATCGAGTGAGAGCCGGACCC GCCACCTCCGGACCCGCCACCTCCG GACCCGCCACCTCCGGACCCGCCAC CTCCCATGTCTC	CCGGGGCTCCCCGAG GCCGGGCCGG GACACGGCGATGTC	GCTCCCCGAGGCCGG GCCGGGACACGGCGA

* Bold cases indicate sequences encoding Tax180-188 or NLEnv371-379 epitope peptide.

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ISSN: 1742-4690

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