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Figure 2 | Retrovirology

Figure 2

From: Activation and detection of HTLV-I Tax-specific CTLs by Epitope expressing Single-Chain Trimers of MHC Class I in a rat model

Figure 2

Establishment of MOLT-4 cells stably expressing SCTs of RT1.Al. (A) MOLT-4 cells were transfected with various SCT expression vectors. After selection by G418 and cloning, flow cytometric analysis was performed to determine the expression level of RT1.Al on MOLT-4 cells. The percentage of RT1.Al-positive cells is indicated in each part. (B and C) The MOLT-4 cells expression with indicated SCTs were incubated with a Tax-specific CD8+ T cell line, 4O1/C8. Production of IFN-γ (B) and TNF-α (C) in the supernatants was then measured by ELISA after 24 hours of culture. The data represent the mean ± the SD of triplicate wells. Similar results were obtained in two independent experiments.

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