Subcellular localization of eGFP or mCherry tagged Vpr by confocal microscopy. HeLa cells were co-transfected with 0.5 μg of each plasmid and 0.5 μg pcDNA3. Cells were observed by confocal microscopy 24 h post transfection. Each panel shows the major phenotype. (A) mCherry images with excitation at 568 nm and emission at 580 to 700 nm. (B) eGFP images with excitation at 488 nm and emission at 500 to 550 nm. Note the intracellular redistribution of eGFP and mCherry upon fusion with Vpr.