Figure 1
From: Homonuclear 1H NMR and circular dichroism study of the HIV-1 Tat Eli variant
Trans-activation assay with HeLa cells transfected with a HIV-1 LTR lacZ construct. (A) The histograms show the trans-activation observed with synthetic Tat Eli using four different concentrations: 2 μM, 1 μM, 0.5 μM, and 0.25 μM. Without Tat, there is a basal expression of β-galactosidase as indicated with control. Error bars represent the standard deviation measured between two independent experiments carried out in triplicate. (B) The histograms show the fold difference in trans-activational activity observed with synthetic Tat Eli and synthetic Tat HXB2(86) compared with recombinant Tat at 50 nM, with recombinant Tat activity set at 1. Error bars represent the standard deviation measured between two independent experiments carried out in triplicate.