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Figure 1 | Retrovirology

Figure 1

From: The histone chaperone protein Nucleosome Assembly Protein-1 (hNAP-1) binds HIV-1 Tat and promotes viral transcription

Figure 1

Identification of Tat-interacting proteins by mass spectrometry. A. Flag-immunoprecipitated material from Tat-Flag- and mock-transfected HEK 293T cells was resolved by 6–10% gradient SDS-PAGE gel, followed by silver staining. Protein bands present exclusively in the sample transfected with Tat-Flag were excised from the gel and their identification attempted by ESI-MS/MS. The identified proteins, in addition to hNAP-1 and Tat-Flag, are indicated (1: B23/nucleophosmin; 2: pre-mRNA splicing factor SF2p32 – Tat-associated protein TAP; 3: ribosomal protein S4). B. Amino acid sequence of the human NAP-1 protein (locus NP_631946) – 391 aa. The underlined amino acid sequences correspond to peptides obtained from MS/MS analysis of three independent preparations (P = 7.8 × 10-19).

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Retrovirology

ISSN: 1742-4690

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