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Figure 3 | Retrovirology

Figure 3

From: A single site for N-linked glycosylation in the envelope glycoprotein of feline immunodeficiency virus modulates the virus-receptor interaction

Figure 3

Infection of the feline cell lines AH927-FX4P [81] (A) and MCC [27] (B) stably-transduced with retroviral vectors encoding full-length fCD134 (CD134), CRD1 of fCD134 in the context of hCD134 (CRD1) or vector only control (CON). Infection of both cell lines expressing CRD1 was enhanced significantly in the T271I and Δ2N Envs. Histograms display the mean +/-SE (n = 3), arrows show P-values, t-test. C.) Syncytium formation in AH927-FX4P cells expressing feline CD134 (CD134), or feline CD134 CRD1/human CD134 chimera (CRD1). Cells were transfected with eukaryotic expression vectors bearing the GL8 or CPG41 envs carrying the WT, T271I, N342Y or Δ2N sequences. Cells were fixed and stained at 48 hours post-transfection, representative fields are shown.

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