Figure 3From: Nuclear import of Avian Sarcoma Virus integrase is facilitated by host cell factorsNuclear import of GST-NLS substrates in digitonin-permeabilized HeLa cells. GST-NLS fusion proteins were incubated in digitonin permeabilized HeLa cells for 30 min at 37°C prior to fixation with paraformaldehyde and staining with fluorescent antibody against GST. Left column panels are import without added cytosol and right column panels with added HeLa cytosol extracts.Back to article page