Figure 4

Deletions or substitutions of specific amino acids in P^BLV-391 have a detrimental effect on inhibitory activity. Syncytium interference assays using BLV envelope-expressing HeLa cells as effectors. (A) The inhibitory properties of P^BLV-391, P^BLV-ΔN, P^BLV-ΔC and the P^cr-400 control were examined. (B) The activity of P^BLV-391, the derivative P^BLV-ΔCCF, and the control peptide P^cr-400 were compared. (C) The activity of P^BLV-391 was compared to Bio-P^BLV-391^Ar a biotinylated peptide recovered from the flow-through of an amylose column (see methods), Bio-P^BLV-391^Sd the same peptide depleted over a streptavidin column (volumes of column buffer equal to those required to give the specified concentrations of Bio-P^BLV-391^Ar were used), and the control peptide C34. (D) The inhibitory properties of P^BLV-391, P^BLV-L/A, P^BLV-L404/410A and the control P^cr-400 were compared. Syncytia were counted in 10 low-power light microscope fields. Data points show the mean ± SD of triplicate assays.