Figure 1
From: Multiple independent origins of a protease inhibitor resistance mutation in salvage therapy patients
Design and test of the L90M allele-specific PCR protocol. A. Alignment of AK90m primer with mutant L90M and wild-type L90 region of protease gene (subtype B consensus). B. PCR amplification using L90M specific primer of serial log dilutions of L90 (AKp136) and L90M (AKp140) plasmid derived generic gag-pro amplicons. C. Sensitivity of L90M allele specific PCR using serial dilution of AKp140 derived amplicon in a constant amount of AKp136 derived L90 amplicon. Ratio of L90M to L90 amplicons DNA is shown