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Table 1 Overview of CycT1 mutants used in this study.

From: Dominant negative mutant Cyclin T1 proteins inhibit HIV transcription by specifically degrading Tat

Regions Helix# Amino acid positions # of clones Activity in murine cells (*1)
     >100% 50–100% <50%
Region 1 2 58–65 29 4 14 11
Region 2 2 67–71 26 3 10 13
Region 3 3 88–91 16 2 5 9
Region 4 3 93–96 16 3 11 2
Region 5 4 104–108 5 0 0 5
Region 6 5 132–137 3 0 1 2
Region 7 5 139–143 9 0 0 9
Region 8 5 145–148 2 0 0 2
Region 9 1' 149–152 9 3 4 2
total    115 15 45 55
  1. *1: Tat transactivation obtained with wt human CycT1 is set as 100%