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Table 2 Fluorescence anisotropy decay parameters of apo-Tat and holo-Tata

From: Modulation of microtubule assembly by the HIV-1 Tat protein is strongly dependent on zinc binding to Tat

 

θ1 (ns)

β1 (%)

θ2 (ns)

β2 (%)

Apo-Tat

0.28 ± 0.03

42 ± 3

2.0 ± 0.2

58 ± 3

Holo-Tat

0.24 ± 0.07

43 ± 6

2.8 ± 0.4

57 ± 6

  1. aExperimental conditions were as in Table 1. The correlation times, θ i , and relative amplitudes, β i , are expressed as means for at least three experiments.